Exploring the antimicrobial efficacy of Manuka honey against multidrug-resistant and extensively drug-resistant Salmonella Typhi causing septicemia in Pakistan
Iqra Bashir a , Muhammad Hidayat Rasool a , Muhammad Shafique a , Kokab Jabeen b & Muhammad Usman Qamar * a
a
Institute of Microbiology, Faculty of Life Sciences, Government College University Faisalabad, Faisalabad, 38000, Pakistan
b
Department of Pathology, Ameer ud Din Medical College/Lahore General Hospital, Lahore, Pakistan
* CONTACT: mailto:musmanqamar@gcuf.edu.pk
Abstract
Aim To determine the efficacy of manuka honey against multidrug-resistant (MDR) and extensively drug-resistant (XDR) clinical strains of SalmonellaTyphi.
Materials & methods: Clinical isolates were processed using the Bactec blood culture system, identification and antibiogram by Vitek 2 and antibiotic resistance genes through polymerase chain reaction (PCR). Microbroth dilution assays evaluated the antibacterial activity of manuka honey.
Results MDR and XDR-S. Typhi was susceptible to azithromycin. These strains carried the H58, gyrA, gyrB, blaCTX-M-15 , and blaTEM-1 genes. At 100% honey, the zone of inhibition for MDR (15–23 mm) and XDR (15–24 mm) strains. 18/50 MDR and 14/50 XDR strains inhibited at 3.125 v/v% killed at 6.25 v/v% concentration respectively.
Conclusion Manuka honey could be an alternative option for treating S. Typhi infections.
Keywords: antimicrobial-resistant genes, manuka honey, MDR, Salmonella Typhi, septicemia, XDR.
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